The Future of "Medicine"
Cancer genes switched off in humans
For the first time, researchers have used short sequences of RNA that can effectively treatin people by silencing specific genes behind tumour production.
Mark Davis from the California Institute of Technology in Pasadena and his colleagues have used the technique, called RNA interference (RNAi), to deliver particles containing such sequences to patients with the skin cancer melanoma.
When analysing biopsies of the tumours after treatment, they found that the particles had inhibited expression of a key gene, called RRM2, needed for the cancer cells to multiply.
The researchers created the particles from two polymers plus a protein that binds to receptors on the surface of cancer cells and pieces of RNA called small-interfering RNA, or siRNA, designed to stop the RRM2 gene from being translated into protein.
The siRNA works by sticking to the messenger RNA (mRNA) that carries the gene's code to the cell's protein-making machinery and ensuring that enzymes cut the mRNA at a specific spot.
When the components are mixed together in water, they assemble into particles about 70 nanometres in diameter. The researchers can then administer the nanoparticles into the bloodstream of patients, where the particles circulate until they encounter 'leaky' blood vessels that supply the tumours with blood.
The particles then pass through the vessels to the tumour, where they bind to the cell and are then absorbed. Once inside the cell, the nanoparticles fall apart, releasing the siRNA. The other parts of the nanoparticle are so small, they pass out of the body in urine.
"It sneaks in, evades the immune system, delivers the siRNA, and the disassembled components exit out," Nature quoted Davis as saying.
When researchers analysed tumour samples from three of the patients who volunteered samples, they found fragments of the mRNA in exactly the length and sequence they would expect from the design of their siRNA.
And in at least one patient, the levels of the protein were lower than they were in samples of the tumours taken before treatment.
They also found that patients who were given higher doses had higher levels of siRNA in their tumours. "The more we put in, the more ends up where they are supposed to be, in tumour cells," said Davis.
Davis says that by targeting specific genes he hopes these treatments will not have major side effects. "My hope is to make tumours melt away while maintaining a high quality of life for the patients. We're moving another step closer to being able to do that now," he said.
The study has been published in Nature.